Optimization of Process Parameters in Submerged Fermentation for Lipase Production from Soil Fungal Isolate

  • Arun kumar Sharma Dept. of Bioscience and Biotechnology, Banasthali University, Rajasthan
  • Sapna Kumari Department of Bioscience and Biotechnology, Banasthali University, Rajasthan, India
  • Vinay Sharma Department of Bioscience and Biotechnology, Banasthali University, Rajasthan, India
  • Jyoti Saxena Department of Biochemical Engineering, Bipin Tripathi Kumaon Institute of Technology, Dwarahat, Uttrakhand.


Lipases are very important industrial enzymes. They are used in number of industries including paper, detergent, food, dairy, pharmaceutical, diagnostic centre, cosmetic etc.  By considering immense applications of lipases, an attempt was made to optimize lipase production in submerged fermentation (SmF) from a fungal isolate. In the present study, various parameters such as carbon source, nitrogen source, pH, temperature and incubation time were optimized for a lipolytic fungal strain named as S2St1, previously isolated from mustard field soil sample, for lipase production in SmF. Maximum activity of lipase (157.28±4.26 U/ml/min) was obtained at 28 ºC, after 7 days of incubation in the medium containing mixture of fructose (0.5% w/v) + coconut oil (1% v/v) as carbon source and peptone (4% w/v) + (NH4)2SO4 (0.1% w/v) as nitrogen source. The present study reveals that coconut oil was found to be best among other lipid carbon source for lipase production, which indicates that presence of inducer (oil) is required for maximum lipase activity. Lipase activity was also found better at 50 ºC (65.77±5.39 U/ml/min), indicating superior thermo-stability of fungal strain for lipase production.

Author Biography

Arun kumar Sharma, Dept. of Bioscience and Biotechnology, Banasthali University, Rajasthan
Assistant Professor


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How to Cite
Sharma, A., Kumari, S., Sharma, V. and Saxena, J. 2017. Optimization of Process Parameters in Submerged Fermentation for Lipase Production from Soil Fungal Isolate. Journal of Biological Sciences and Medicine. 3, 2 (Jun. 2017), 1-8.
Research Articles