Hepatic changes in albino rat induced by the inhalation of Ammonia gas
In the present study albino rats, were exposed to two different concentrations (50 and 150ppm) of NH3 for one hour per day for different time duration (15 and 30 day). The histopathological studies to assess the ammonia toxicity in terms of developed vascular changes were carried out. The ammonia gas exhibited multiple histological abnormalities in liver which include necrosis and darkening of nuclei (apoptotic cells). The histopathological necrosis and degenerative changes in the liver tissue were accompanied by the alteration in the level of blood enzymatic parameters. The activity of marker enzymes of liver function (SGOT and SGPT) was estimated in the blood serum collected from control and exposed rats. A significant elevation in the activity of these enzymes was recorded in serum of treated rats which indicates their involvement in developing necrotic lesions in the liver. The SGOT activity was noted to be relatively higher in comparison to SGPT. Thus, changes in the activities of serum enzymes (SGOT and SGPT) may be a potential biomarker for NH3 induced hepatotoxicosis ultimately affecting the general health by altering the functional and structural integrity of liver.
Campbell JW (1973) Nitrogen excretion in: Comparative animal physiology’ (Ed Prosser. CI) Saunders Co., London 279-316
EFMA (European Fertilizer Manufacturers’ Association) (2000) Production of Ammonia. Booklet No. 1 of 8 Ave. E van Nieuwenhuyse 4 B-1160 Brussels, Belgium.
Kosenko E, Kaminski Y, Lopata O, Muravyov N, Felipo V (1999) Blocking NMDA receptors prevents the oxidative stress induced by acute ammonia intoxication. Free Rad Biol Med 26 (11-12): 1369–1374
Kosenko E, Kaminsky Y, Kaminsky, Valencia M, Lee L, Hermenegildo C, Felipo V (1997) Superoxide production and antioxidant enzymes in ammonia intoxication in rats. Free Radical Res 27: 637-644
Lemberg A, Fernandez MA (2009) Hepatic encephalopathy, ammonia, glutamate, glutamine and oxidative stress. Ann Hepatol 8: 95–102
Lena PJ, Subramanian P (2004) Effects of melatonin on the levels of antioxidants and lipid peroxidation products in rats treated with ammonium acetate. Pharmazie 59 (8): 636–639
Norenberg MD, Ramma Rao KV, Jayakumar AR (2005) Mechanisms of ammonia induced astrocyte swelling. Metab Brain Dis 20: 302–317
Sadasivudu B, Indira Rao T, Murthy CR (1977) Acute metabolic effects of ammonia in mouse brain. Neurochem Res 2: 639- 55
Sireesha A and P Neeraja (2012) Induced ammonia stress on development of albino rat through study of certain biochemical components. Int. J. Adv. Biol. Research, 2(4) 704-707
Sujatha K (2011) Role of Trace elements in hyperammonia in albino rat, M.phil Thesis S.V. University, Tirupati
Visek WJ (1984) Ammonia: Its Effects on Biological Systems, Metabolic Hormones, and Reproduction J Dairy Science 67: 481-498
Walker V (2009) Ammonia toxicity and its prevention in inherited defects of the urea cycle. Diab Obes Metab 1(9): 823– 385
Weatherburn MW (1967) Phenol–hypochlorite reaction for determination of ammonia. Anal Chem 39: 971-974
Weatherby H (1952) Chronic toxicity of ammonia fumes by Inhalation. Pr'oe Soc Exp:t I Dial Mod 81: 300-301
Thenmozhi, AJ, Subramanian P (2011) Antioxidant Potential of Momordica charantia in Ammonium Chloride-Induced Hyperammonemic Rats. Evidence-Based Complementary and Alternative Medicine : eCAM, 612023. http://doi.org/10.1093/ecam/nep227
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