Development and standardization of PCR technique for detection of ciliate parasite Ichthyophthirius multifiliis from freshwater exotic carp Cyprinus carpio and Indian major carp Labeo rohita
Recent growth in aquaculture and fisheries has reshaped the methodology of treatment and diagnosis of diseases. Molecular techniques, mainly nucleic acid-based testing has become a crucial diagnostic tool for genetic disease and infectious processes. Their application in monitoring several pathogens related risks and identification of emerging pathogens is quite useful. Polymerase chain reaction (PCR) is an efficient and rapid method to increase a small quantity of DNA into ample quantity, for the detection of pathogens. The applications of these technologies are limitless and improving progressively. Validations of molecular tools provide essential underpinnings for a successful application in biological studies. Therefore, we have developed and standardized the PCR based assay for the detection of Ichthyophthrius multifilliis which is a fatal parasite of freshwater fishes, distributed worldwide. The present article reports some new sets of primers for the ciliate parasite I. multifilliis.
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