Alternations of selenium and malondialdehyde status in chronic kidney disease

  • Myat Mon Khine Department of Biochemistry, University of Medicine-2, Myanmar
  • Htay Htay Department of Biochemistry, University of Medicine-2, Myanmar
  • Nyo Nyo Department of Biochemistry, University of Medicine-2, Myanmar


Chronic kidney disease (CKD) is a major public health burden worldwide. CKD patients have cardiovascular risk factors such as diabetic mellitus and hypertension. These factors are associated with oxidative stress which accelerates renal injury in CKD patients. This study aimed to determine selenium (antioxidant trace element) and malondialdehyde (MDA, oxidative stress marker) in patients with CKD. Serum selenium and MDA levels are analyzed in CKD patients (n=20) compared with values observed in control (n=20). Serum urea, creatinine and MDA levels were determined with spectrophometric methods and serum selenium level was measured by atomic absorption spectrophotometry. Serum selenium and MDA in CKD patients showed significant differences in comparison with control – MDA 2.42 ± 0.57 vs. 0.98 ± 0.09 µmol/L (p<0.001) and selenium 81.52 ± 10.24 vs. 105.46 ± 10.53 µg/L (p<0.001). A significant negative correlation was observed between serum selenium and MDA in CKD patients (r = - 0.88, p < 0.0001), while there was a significant positive correlation of selenium level with creatinine clearance in CKD (r = 0.714, p <0.001). There is imbalance between oxidative stress and antioxidant status in CKD. We suggest MDA could be used as oxidative marker for progressive kidney diseases.


Ceballos-Picot I, Witko-Sarsat V, Merad-Boudia M, Nguujen A, Thevenin M, Jaudon M et al (1996) Glutathione peroxidase is the marker of oxidative stress in chronic renal failure. Free Radi Biol Med 21(6):845-854

Esterbauer H, Cheeseman K (1990) Determination of aldehydic lipid peroxidaiton products: Malondialdehyde and 4-hydroxynonenal. Method Enzymol 186:408-409

Gansevoort R, Correa-Rotter R, Hemmelgarn B, Jafar T, Heerspink H, Mann, et al (2013) Chronic kidney disease and cardiovascular risk: epidemiology, mechanisms, and prevention. Lancet 382:339-352. doi:10.1016/S0140-6736(13)60595-4

Hawk P, Oser B, Summerson W (1957)Prac Physiol Chem (13 ed.) New York: McGraw Hill.

Hill NF, Oke J, Hirst J, O'Callaghan C, Lasserson D, Richard-Hobbs F (2016) Global prevalence of chronic kidney disease - a systemic review and meta-analysis. PLoS One e0158765. doi:10.1371/journal.pone.0158765

Iglesias P, Segas R, Romero S, Diez J (2013) Selenium and kidney disease. J Nephrol 26(2):266-272

Kaminska-Galwas B, Grzerzczak W, Jedryczka A, Pachelski J (1993) Levels of selenium, zinc, copper and nickel in serum of patients treated for chronic renal failure. Pol Arch Med Wewn 89(5):368-376

KDOQI (2002) Chronic Kidney Disease: evaluation, classfication, and stratification. KDOQI. Retrieved from

Marsh W, Fingerhut B, Miller H (1965) Determinaiton of serum urea by diacetyl monoxime methods. Clin Chem 11:624

MassyZ, Stenvinkel P, Drueke T (2009) The role of oxidative stress in chronic kidney disease. Semin Dial 22(4):405-408

Miyata T, Kurokania K, Van Y, De-strihou C (2000) Relevance of oxidative stress and carbonyl stress to long-term uremic complications. Kidney Int Suppl 58(6):s120-s125

Morena, Marion, Cristol, Jeen-Paul, Senecal, Lyne, et al (2002) Oxidative stress in haemodialysis patients. Kidney Int Suppl 61:s109-s114

Nath K, Salahudcen A (1990) Induction of renal growth and injury in the intact rat kidney by dietary deficiency of antioxidants. J Clin Invest 86(4):1179-1192

Oster O, Prellwitz W (1982) A methodological comparism of hydride and carbon furnace atomic absorption spectroscopy for the determination of selenium in serum. Clin Chem Acta 124:277-291

Oster O, Prellwit W (1990) The renal excretion of selenium. Biol Trace Elem 24(2):119-146

Rayman MP (2000) The importance of selenium to human health. Lancet 356:233-241

Reddi A, Bollineni J (2001) Selenium deficient diet induces renal oxidative stress in normal and diabetic rats. Kid Int 59(4):1342-1353

Schweizer U, Streckfuss F, Pelt P, Carlson B, Hatfield DI, et al (2005) Hepatically derived selenoprotein P is a key factor for kidney but not for brain selenium supply. Biochem J 386:221-226

Wasowicz W (1987) Selenium concentration in the blood and in urine of a healthy Polish sub-population. J Clin Chem Clin Biochem 25:409-412

Zachara BA, Salak A, Koterska D, Manitius J, Wasowicz W (2004) Selenium and glutathione peroxidases in blood of patients with different stages of chronic renal failure. J Trace Elem Med Biol 17:291-299

How to Cite
Khine, M., Htay, H. and Nyo, N. 2018. Alternations of selenium and malondialdehyde status in chronic kidney disease. Journal of Biological Sciences and Medicine. 4, 3 (Oct. 2018), 1-4.
Research Articles