Optimization of Process Parameters in Submerged Fermentation for Lipase Production from Soil Fungal Isolate

Arun kumar Sharma, Sapna Kumari, Vinay Sharma, Jyoti Saxena

Abstract


Lipases are very important industrial enzymes. They are used in number of industries including paper, detergent, food, dairy, pharmaceutical, diagnostic centre, cosmetic etc.  By considering immense applications of lipases, an attempt was made to optimize lipase production in submerged fermentation (SmF) from a fungal isolate. In the present study, various parameters such as carbon source, nitrogen source, pH, temperature and incubation time were optimized for a lipolytic fungal strain named as S2St1, previously isolated from mustard field soil sample, for lipase production in SmF. Maximum activity of lipase (157.28±4.26 U/ml/min) was obtained at 28 ºC, after 7 days of incubation in the medium containing mixture of fructose (0.5% w/v) + coconut oil (1% v/v) as carbon source and peptone (4% w/v) + (NH4)2SO4 (0.1% w/v) as nitrogen source. The present study reveals that coconut oil was found to be best among other lipid carbon source for lipase production, which indicates that presence of inducer (oil) is required for maximum lipase activity. Lipase activity was also found better at 50 ºC (65.77±5.39 U/ml/min), indicating superior thermo-stability of fungal strain for lipase production.


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