Association of IL-1RN polymorphism with diabetes type 2 in Central Indian Population

Udita Singh, Pallavi Indurkar, Arti Saxena, Pankaj Mishra, Arvind K Tripathi

Abstract


The interleukin-1 receptor antagonist (IL-1RA) is a protein encoded by the IL1RN gene in human. In present study, anti-inflammatory gene; IL1RN has been studied for their association with diabetes type 2. A number of 190 diabetics and 210 healthy controls of central India were recruited for this study and anthropometric and lifestyle data were collected during sampling. Genetic polymorphism study of IL-1β gene was undertaken using PCR-RFLP, meanwhile IL-4 polymorphism was investigated using PCR-VNTR (Variable number of tandem repeats) analysis. Genotype, allele frequencies and carriage rates of IL-1β and IL-4 polymorphisms were recorded during this study. Diabetic patients had higher levels of fasting plasma glucose (P<0.0001), HbA1c (P<0.0001) and Post prandial glucose (P<0.0001) compared to control subjects. Nominal difference was observed for LDL-C (P=0.0462) and triglyceride (P=0.0024). Diabetic patients had higher levels of weight of women (P=0.0024), men (P=0.0157) and Body Mass Index (BMI) of women (P=0.0388), Waist Hip Ratio (WHR) in women (P<0.0001) and WHR in men (P=0.0147). Our findings suggested carriage of genotype I/II and II/II (p=0.0002; OR=2.194, 95% CI 1.455- 3.310 and p=0.0772; OR=1.80, 95% CI 0.9434- 3.434 respectively) were strongly associated with diabetes susceptibility.


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