Development and standardization of PCR technique for detection of ciliate parasite Ichthyophthirius multifiliis from freshwater exotic carp Cyprinus carpio and Indian major carp Labeo rohita

Naireen Fariya, Rehana Abidi, U. K. Chauhan

Abstract


Recent growth in aquaculture and fisheries has reshaped the methodology of treatment and diagnosis of diseases. Molecular techniques, mainly nucleic acid-based testing has become a crucial diagnostic tool for genetic disease and infectious processes. Their application in monitoring several pathogens related risks and identification of emerging pathogens is quite useful. Polymerase chain reaction (PCR) is an efficient and rapid method to increase a small quantity of DNA into ample quantity, for the detection of pathogens. The applications of these technologies are limitless and improving progressively. Validations of molecular tools provide essential underpinnings for a successful application in biological studies. Therefore, we have developed and standardized the PCR based assay for the detection of Ichthyophthrius multifilliis which is a fatal parasite of freshwater fishes, distributed worldwide. The present article reports some new sets of primers for the ciliate parasite I. multifilliis.


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References


Abidi R, Fariya N, Chauhan UK (2015) Development and standardization of PCR technique to detect myxozoan parasites and its use in identification of two exotic Myxobolus species from Indian catfish Clarias batrachus (linn.) Int J fisheries Aquat Studies 2(4): 374- 377

Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402

Bustin SA (2010) Why the need for QPCR publication guidelines? - The case for MIQE. Methods 50(4):217-226

Chen W, Sun HY, XieMQ, BaiJS, Zhu XQ,Li AX (2008) Development of specific PCR assays for the detectionofCryptocaryonirritans. Parasitol Res 103:423–427

Cheng CM, Van Khanh T, Lin W, Ruby RM (2009) Inter laboratory validation of a real- time PCR 24-hour rapid method for detection of Salmonella in foods. J Food Prot 72(5): 945-951

Colombo FA, Vidal JE, Penalva de Oliveira AC, Hernandez AV, Filho FB, Nogueira RS, Focaccia R, Pereira-Chioccola1 VL (2005) Diagnosis of Cerebral Toxoplasmosis in AIDS Patients in Brazil: Importance of Molecular and Immunological Methods Using Peripheral Blood Samples. J Clinical Microbiol 43(10): 5044–5047

Conraths FJ, Schares G (2006) Validation of molecular- diagnostic techniques in the parasitological laboratory. Vet Parasitol 136(2):91-98

Dickerson HW (2006) Ichthyophthirius multifiliis and Cryptocaryon irritans (Phylum, Ciliophora). In: P. T. K. Woo (Ed.), Fish Diseases & Disorders. Vol.1 Protozoan & Metazoan Infections. CAB INTERNATIONAL Walling ford, U.K, pp. 116-153

Field KG, Olsen GJ, Lane DJ, Giovannoni SJ, Ghiselin MT, Raff EC, Pace NR, Raff RA (1988) Molecular phylogeny of the animal kingdom. Science 239 (4841 Pt 1):748-53

Girones R, Ferrus MA, Alonso JL, Manzano RJ (2010) Molecular detection of pathogens in water - The pros and cons of molecular techniques. Water Research 44:4325-4339

Hawk DD (2004) Political and Legal Consequences of Testing and the History of PCR'S accepted uses. (In, Special Session)

Hillis DD (1987) Molecular versus Morphological approaches to systematics. Ann Rev Ecol Syst 18:23- 42

Jousson O, PrettiC, Di Bello D, Cognetti-Varriale AM (2005) Non-invasive detection and quantification of the parasitic ciliate Ichthyophthirius multifiliis by real-time PCR. Diseases of aquatic organisms 65: 251–255

Mayr E, Ashlock PD (1991) Taxonomic Characters In: Principles of Systematic Zoology, 2nd Edition. McGraw-Hill, New York. pp 159-194

Molnár K, Székely C (2003) Infection in the fins of the goldfish Carassius auratus caused by Myxobolus diversus (Myxosporea). Folia Parasitol (Praha) 50(1):31-36

Sen K, Schable NA, Lye DJ (2007) Development of an internal control for evaluation and standardization of a quantitative PCR assay for detection of Helicobacter pylori in drinking water. Appl Environ Microbiol 73:7380–7387

Shalaby SI, Ibrahim MM (1988) The relationship between the monogenetic trematodes Cichlidogyrus tub icirrusmagnus first record in Egypt and morphological lesions of gills among Tilapia nilotica. Egyptian J Com Path Clinical Path 1(9): 116-126

Wong SW, Leong TS (1987) Current fish disease problems in Malaysia. In: Fish quarantine & fish diseases in Southeast Asia (1986 update). Report of Asian Fish Health Network Workshop held in Manila, Philippines, 1986 Asian Fishery Society in association with IDRC. pp. 12


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