Significant correlation between high motility and P450- aromatase expression in human spermatozoa - an immunochemistry based study
Study was planned to evaluate the correlation between sperm motility, an important functional parameter of spermatozoa and aromatase P450 expression in human spermatozoa. Total 70 random seminal samples were included in the present study. Seminal samples from men undergoing seminal investigations for couple infertility were collected. Immunochemical localization with specific monoclonal antibodies against the P450- aromatase enzyme with washed seminal slides was carried out and the results correlated with spermatozoon motility evaluation retrospectively. Expression of aromatase P450 was assessed by assigning 1+ to least and 4+ to highest. The 94.6% cases of spermatozoa showing immunochemical positive grading >3+ for -aromatase P450, had motility greater than 70%. Similarly 94% cases show <2+ intensity of aromatase P450 expression on spermatozoa found with motility of less than 70%. The study suggests that the spermatozoa aromatase P450 produced estradiol might be play an important role in spermatozoa motility.
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