Significant correlation between high motility and P450- aromatase expression in human spermatozoa - an immunochemistry based study
Study was planned to evaluate the correlation between sperm motility, an important functional parameter of spermatozoa and aromatase P450 expression in human spermatozoa. Total 70 random seminal samples were included in the present study. Seminal samples from men undergoing seminal investigations for couple infertility were collected. Immunochemical localization with specific monoclonal antibodies against the P450- aromatase enzyme with washed seminal slides was carried out and the results correlated with spermatozoon motility evaluation retrospectively. Expression of aromatase P450 was assessed by assigning 1+ to least and 4+ to highest. The 94.6% cases of spermatozoa showing immunochemical positive grading >3+ for -aromatase P450, had motility greater than 70%. Similarly 94% cases show <2+ intensity of aromatase P450 expression on spermatozoa found with motility of less than 70%. The study suggests that the spermatozoa aromatase P450 produced estradiol might be play an important role in spermatozoa motility.
Aquila S, Sisci D, Gentile M, Carpino A, Middea E, Catalano S, Rago V, Andò S (2003). Towards a physiological role for cytochrome P450 aromatase in ejaculated human sperm. Hum Reprod 18(8):1650-1659
Aquila S, Sisci D, Gentile M, Middea E, Siciliano L, Andò S (2002) Human ejaculated spermatozoa contain active P450 aromatase. J Clin Endocrinol Metab 87(7):3385-3390.
Carreau S, de Vienne C, Galeraud-Denis I (2008) Aromatase and estrogens in man reproduction: a review and latest advances. Adv Med Sci 53(2):139-44
Carreau S, Silandre D, Bourguiba S, Hamden K, Said L, Lambard S, Galeraud-Denis I, Delalande C (2007) Estrogens and male reproduction: a new concept. Braz J Med Biol Res 40(6):761-768.
Carreau S, Wolczynski S, Galeraud-Denis I (2010) Aromatase, oestrogens and human male reproduction. Philos Trans R Soc Lond B Biol Sci 365(1546):1571-1579.
Eddy EM, Washburn TF, Bunch DO, Goulding EH, Gladen BC, Lubahn DB, Korach KS (1996) Targeted disruption of the estrogen receptor gene in male mice causes alteration of spermatogenesis and infertility. Endocrinology 137(11):4796-4805.
Fisher CR, Graves KH, Parlow AF, Simpson ER (1998) Characterization of mice deficient in aromatase (ArKO) because of targeted disruption of the cyp19 gene. Proc Natl Acad Sci U S A 95(12):6965-6970.
Fraser LR, Beyret E, Milligan SR, Adeoya-Osiguwa SA (2006) Effects of estrogenic xenobiotics on human and mouse spermatozoa. Hum Reprod 21(5):1184-1193.
Galeraud-Denis I, Lambard S, Carreau S (2007) Relationship between chromatin organization, mRNAs profile and human male gamete quality. Asian J Androl 9(5):587-92
Gustafson ML, Donahoe PK (1994) Male sex determination: current concepts of male sexual differentiation." Annu Rev Med 45:505-524
Hess RA, Bunick D, Lee KH, Bahr J, Taylor JA, Korach KS, Lubahn DB (1997). A role for oestrogens in the male reproductive system. Nature 390(6659):509-512
Hsu SM, Raine L, Fanger H (1981) Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem 29(4):577-580
Idaomar M, Guerin JF, Lornage J, Czyba JC (1989) Stimulation of motility and energy metabolism of spermatozoa from asthenozoospermic patients by 17 beta-estradiol. Arch Androl 22(3):197-202
Janulis L, Bahr JM, Hess RA, Janssen S, Osawa Y, Bunick D (1998) Rat testicular germ cells and epididymal sperm contain active P450 aromatase. J Androl 19(1):65-71
Krege JH, Hodgin JB, Couse JF, Enmark E, Warner M, Mahler JF, Sar M, Korach KS, Gustafsson JA, Smithies O (1998) Generation and reproductive phenotypes of mice lacking estrogen receptor beta. Proc Natl Acad Sci U S A 95(26):15677-15682.
Kwon S, Hess RA, Bunick D, Nitta H, Janulis L, Osawa Y, Bahr JM (1995) Rooster testicular germ cells and epididymal sperm contain P450 aromatase. Biol Reprod 53(6):1259-1264
Lambard S, Galeraud-Denis I, Bouraïma H, Bourguiba S, Chocat A, Carreau S (2003) Expression of aromatase in human ejaculated spermatozoa: a putative marker of motility. Mol Hum Reprod 9(3):117-124
Lambard, S., I. Galeraud-Denis, et al. (2004). "Analysis and significance of mRNA in human ejaculated sperm from normozoospermic donors: relationship to sperm motility and capacitation." Mol Hum Reprod 10(7): 535-41.
Sharma Pankaj K., K. K., Jayaraman S (2017) "Immunohistochemical Localization of Estrogen Receptors in Human Spermatozoa." International Journal of Research Studies in Biosciences 5(11): 5
Pentikäinen V, Erkkilä K, Suomalainen L, Parvinen M, Dunkel L (2000) Estradiol acts as a germ cell survival factor in the human testis in vitro. J Clin Endocrinol Metab 85(5):2057-2067
Rago V, Bilińska B, Palma A, Andò S, Carpino A (2003) Evidence of aromatase localization in cytoplasmic droplet of human immature ejaculated spermatozoa. Folia Histochem Cytobiol 41(1):23-27
Kirsten M. Robertson, Liza O’Donnell, Margaret E. E. Jones, Sarah J. Meachem, Wah Chin Boon, Carolyn R. Fisher, Kathy H. Graves, Robert I. McLachlan, Evan R. Simpson (1999) Impairment of spermatogenesis in mice lacking a functional aromatase (cyp 19) gene. Proc Natl Acad Sci U S A 96(14):7986-7991.
Turner KJ, Macpherson S, Millar MR, McNeilly AS, Williams K, Cranfield M, Groome NP, Sharpe RM, Fraser HM, Saunders PT (2002) Development and validation of a new monoclonal antibody to mammalian aromatase. J Endocrinol 172(1):21-30
WHO (2010). WHO laboratory manual for the Examination and processing of human semen. Switzerland, WHO Press, World Health Organization, Switzerland.
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