Hepatic changes in albino rat induced by the inhalation of Ammonia gas
In the present study albino rats, were exposed to two different concentrations (50 and 150ppm) of NH3 for one hour per day for different time duration (15 and 30 day). The histopathological studies to assess the ammonia toxicity in terms of developed vascular changes were carried out. The ammonia gas exhibited multiple histological abnormalities in liver which include necrosis and darkening of nuclei (apoptotic cells). The histopathological necrosis and degenerative changes in the liver tissue were accompanied by the alteration in the level of blood enzymatic parameters. The activity of marker enzymes of liver function (SGOT and SGPT) was estimated in the blood serum collected from control and exposed rats. A significant elevation in the activity of these enzymes was recorded in serum of treated rats which indicates their involvement in developing necrotic lesions in the liver. The SGOT activity was noted to be relatively higher in comparison to SGPT. Thus, changes in the activities of serum enzymes (SGOT and SGPT) may be a potential biomarker for NH3 induced hepatotoxicosis ultimately affecting the general health by altering the functional and structural integrity of liver.
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