Evaluation of allozyme markers in housefly population from Mirzapur, India
Genetic variation in house fly (Musca domestica) populations from Mirzapur was analyzed at five gene enzyme system namely acid phosphatase (ACPH), alkaline phosphatase (APH), malate dehydrogenase (MDH), malic enzyme (ME) and aldehyde oxidase (AO). Eight out of the nine enzyme loci were polymorphic. The present study shows that populations from Mirzapur reveals that almost all the polymorphic loci were highly differentiated and the Fis values observed indicate a high degree of inbreeding among the populations.
Allendorf FW, Leary RF (1986) Heterozygosity and fitness in natural populations of animals. Pages 57–76 in M. E. Soulé, editor. Conservation Biology: the science of scarcity and diversity. Sinauer Associates, Sunderland, Massachusetts
Ayala FJ, Powell JR, Tracey ML, Mourao CA, Perez-Salas S (1972) Enzyme variability in the Drosophila willistoni group IV. Genic variation in natural populations of Drosophila willistoni. Genetics 70: 113-139
Barker JSF, East PD, Weir BS (1986) Temporal and microgeographic variation in allozyme frequencies in a natural population of Drosophila buzzatii. Genetics 112: 577-585
Beardmore J (1983) Extinction, survival, and genetic variation. Pages 125–151 in C. M. Schonewald-Cox, S. M. Chambers, F. MacBryde, and L. Thomas, editors. Genetics and conservation: a reference for managing wild animal and plant populations. Benjamin/Cummings, Menlo Park, California.
Black IV WC, Krafsur ES (1985) Electrophoretic analysis of genetic variability in the house fly (Musca domestica L.) Biochem Genet 23(3-4), 193-203
Black IV, WC, Krafsur ES (1986) Temporal and spatial trends in allozyme frequencies in house fly populations, Musca domestica L. Theor Appl Genet 71: 673-681
Bubliy OA, Kalabushkin BA, Imasheva AG (1999) Geographic variation of six allozyme loci in Drosophila melanogaster: An analysis of data from different continents. Hereditas 130: 25-32
Cummings MA, Krafsur ES (2005) Spatial diversity in mitochondrial cytochrome oxidase in house fly. Med Vet Entomol 19: 53-59
Harry M, Galindez I, Cariou ML (1992) Isozyme variability and differentiation between Rhodinus prolixus, R. robustus and R. pietipes, vectors of chagas disease in Venezuela. Med Vet Entomol 6: 37-43
Hartl DL (2001) A Primer of Population Genetics. Sinauer Associates, Inc. Publishers, Sunderland, MA. 221 pp. £21.95. J Animal Breed Genetics 118: 234
Hederick PW, Ginevan ME, Ewing EP (1976) Genetic polymorphism in heterogeneous environments. Ann Rev Ecol Syst 7: 1-27
Hedrick PW, Miller P (1992) Conservation genetics: techniques and fundamentals. Eco App 2: 30–46
Houle D (1989) Allozyme associated heterosis in Drosophila melanogaster. Genetics 123:789–801
Kimura M (1983) The Neutral Theory of Molecular Evolution, Cambridge University Press, Cambridge
Krafsur ES, Helm JM, Black IV WC (1992) Genetic diversity at electrophoretic loci in the house fly, Musca domestica L. Biochem Genet 30: 317-329
Krafsur ES, Bryant NL, Marquez JG, Griffith NT (2000) Genetic distance among North American, British and West African house fly populations Musca domestica L. Biochem Genet 38: 275-284
Kraushaar U, Goudet J, Blanckenhorn WU (2002) Geographical and altitudinal population genetic structure of two dung fly species with contrasting mobility and temperature preference. Heredity 89: 99-106
Land JV, Van Putten WF, Villarrole H, Kamping A, Van Delden W (2000) Latitudinal variation for two enzyme loci and an inversion polymorphism in Drosophila melanogaster from central and South America. Evolution 54: 201- 213
Lewontin RC and Hubby JL (1966). A molecular approach to the study of genic heterozygosity in natural populations. II. Amount of variation and degree of heterozygosity in natural populations of Drosophila pseudoobscura. Genetics 54: 595-609
Malacrida AR, Guglielmino CR, Gasperi G, Baruffi L, Milani R (1992) Spatial and temporal differentiation in colonizing populations of Ceratitis capitata. Heredity 69: 101-111
Marquez JG, Krafsur ES (2002) Gene flow among geographically diverse house fly populations (Musca domestica, L.): a worldwide survey of mitochondrial diversity. J Hered 93: 254-259
Marquez JG, Moon RD, Krafsur ES (2001) Genetic differentiation among populations of house flies (Diptera: Muscidae) breeding at a muiltiple-barn, egg facility in Central Minnesota. J Med Entomol 38: 218-222
Milankov V, Stamenkovic J, Vujic A, Simic S (2002) Geographic variation of Chelosia vernalis (Fallen, 1817) (Diptera: Syrphidae). Acta Zool Hung 48: 255-267
Narang S (1980). Genetic variability in natural populations, evidence in support of the selectionists view. Experientia 36: 50-51
Nayar JK, Knight JW, Munstermann LE (2003) Temporal and geographic genetic variation in Culex pipiens quinquefasciatus (Diptera: Culicidae) from Florida. J Med Entomol 40: 882-889
Nei M (1972).Genetic distance between populations. American Naturalist 106: 283-292
Murphy RW, Sites JW, Buth DG and Haufler CH (1996) Proteins: isozyme electrophoresis. In: Hills DM, Moritz C., and Mable BK, (eds.), Molecular systematics. Sinauer Associates. Inc. Sunderland, Massachusetts, U.S.A., pp. 655
Reed DH, Frankham R (2001) How closely correlated are molecular and quantitative measures of genetic variation? A meta-analysis. Evolution 55:1095–1103
Santos JMM, Rodriguez GAD, Maia JF, Tadei WP (2005) Variability and genetic differentiation among Anopheles (Ano) intermedius Chagas, 1908 and Anopheles (Ano) mattogrossensis Lutz and Neiva, 1911(Diptera: Culicidae) from the Brazilian Amazon. Mem Inst Oswaldo Cruz 100: 631-637
Sharma AK, Mendki,M.J.; Tikar,S.N.; Chandel,K.; Sukumaran,D., Parashar,B.D.; Vijay Veer, O.P.; Agarwal and Shri Prakash.(2009). Genetic variability in the geographical populations of Culex quinquifasciatus Say (Diptera: Culicidae) from India based on random amplified polymorphic DNA analysis. Acta tropica 112: 71-76
Srivastava S (2009) Genetic variation in Musca (Diptera: Muscidae). D.Phil. Thesis, University of Allahabad, Allahabad, India.
Srivastava S, Singh R, Tewari RR (2012) Temporal variation among populations of house fly Musca domestica (Diptera: Muscidae). Int J Pharma Bio Sci 3 (1): 254-260
Stanger J (1984) Preliminary observations on genetic variation in three colonies of Musca domestica (Diptera: Muscidae) isolated from central Alberta. Quaest Entomol 20: 51-59
Tewari RR, Thakur S (1991) Acid phosphatase isozymes in five species of Sarcophagid flies. Nat Acad Sci Letters 14: 351-354
Tewari RR, Thakur S (1994) Malate dehydrogenase isozymes in five species of Sarcophagid flies (Sarcophagidae: Diptera). Comp Biochem Physiol 109 (B): 567-570
Tripathi M, Tewari RR, Agrawal UR (2010) Genetic variations in housefly Musca domestica L. (Diptera: Muscidae) from Allahabad (India). Proc Nat Acad Sci India Section B 80: 24-29
Tripathi M, Agrawal UR, Tewari RR (2011) Seasonal genetic variation in house fly populations, Musca domestica (Diptera: Mucsidae). Cell Mol Biol 57: 129-134
Tripathi M, Agrawal UR, Tripathi J, Tewari RR (2012) Spatial genetic variation in house fly populations, Musca domestica (Diptera :Mucsidae). Int J Pharm Bio Sci 3(4): 927 – 934
Tripathi M, Agrawal UR and Tripathi J (2015) Electrophoretic Analysis in two groups of enzymes of Musca domestica L. (Diptera: Muscidae). Int Res J Biological Sci 4(10): 20-23
Tsukamoto M (1989) Enhancement of staining intensity of mosquito larva zymograms after electrophoresis. J UOEH 11: 461-479
Tsukamoto M, Horio M (1985) Electrophoretic comparison of the lactate dehydrogenase banding pattern among Japanese mosquito larvae (Diptera: Culicidae). J Med Vet Entomol 22: 491-498
Copyright (c) 2016 Srivastava
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).